Naked droplets for culturing cells

Culturing Uveal Melanoma Cells.

A major challenge in cancer research is the use of appropriate models with which to study a specific biological question. Cell lines have long been used to study cellular processes and the effects of individual molecules because they are easy to use, grow rapidly, produce reproducible results and have a strong track record in research. In uveal melanoma in particular, the absence of animal mode...

Improved methods for culturing rat glomerular cells.

Several investigators [1] have outlined techniques for culturing glomerular cells but many of these methods resulted in mixed cell populations and an inability to passage the cells consistently. There is also a lack of good criteria for cell identification. Previously, Kreisberg, Hoover, and Karnovsky [21 devised techniques in which they were able to isolate and grow pure, homogeneous cultures ...

Culturing intestinal stem cells: applications for colorectal cancer research

Recent advance of sequencing technology has revealed genetic alterations in colorectal cancer (CRC). The biological function of recurrently mutated genes has been intensively investigated through mouse genetic models and CRC cell lines. Although these experimental models may not fully reflect biological traits of human intestinal epithelium, they provided insights into the understanding of inte...

Culturing cells in a mechanically active environment.

Methods for culturing mouse and human embryonic stem cells.

Mouse embryonic stem cells (mESCs) were first derived and cultured almost 30 years ago and ever since have been valuable tools for creating knockout mice and for studying early mammalian development. More recently (1998), human embryonic stem cells (hESCs) have been derived from blastocysts, and numerous methods have evolved to culture hESCs in vitro in both complex and defined media. hESCs are...